(Ebook) Laser Capture Microdissection: Methods and Protocols (Methods in Molecular Biology) by Graeme I. Murray, Stephanie Curran ISBN 9781588292605, 9781592598533, 1588292606, 1592598536

(Ebook) Laser Capture Microdissection: Methods and Protocols (Methods in Molecular Biology) by Graeme I. Murray, Stephanie Curran ISBN 9781588292605, 9781592598533, 1588292606, 1592598536

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip® microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI® approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.