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(Ebook) Molecular Motors Methods and Protocols Methods in Molecular Biology 1st Edition by Ann O Sperry ISBN 1588296652 9781588296658

  • SKU: EBN-2175112
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Instant download (eBook) Molecular Motors: Methods and Protocols (Methods in Molecular Biology Vol 392) after payment.
Authors:Ann O. Sperry
Pages:240 pages.
Year:2007
Editon:1
Publisher:Humana Press
Language:english
File Size:23.51 MB
Format:pdf
ISBNS:9781588296658
Categories: Ebooks

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(Ebook) Molecular Motors Methods and Protocols Methods in Molecular Biology 1st Edition by Ann O Sperry ISBN 1588296652 9781588296658

(Ebook) Molecular Motors Methods and Protocols Methods in Molecular Biology 1st Edition by Ann O Sperry - Ebook PDF Instant Download/Delivery: 1588296652, 9781588296658
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ISBN 10: 1588296652 
ISBN 13: 9781588296658
Author: Ann O Sperry

Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.

(Ebook) Molecular Motors Methods and Protocols Methods in Molecular Biology 1st Table of contents:

  1. Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin Using Co‑Sedimentation and ATPase Assays (Anireddy S.N. Reddy)
  2. In Vitro and In Vivo Analysis of Microtubule‑Destabilizing Kinesins (Jason Stumpff et al.)

  3. Approaches to Kinesin‑1 Phosphorylation (Gerardo Morfini et al.)

  4. Protein Modification to Probe Intradynein Interactions and In Vivo Redox State (Ken‑ichi Wakabayashi et al.)

  5. Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains (Kevin W.–H. Lo & K. Kevin Pfister)

  6. Identification of Motor Protein Cargo by Yeast 2‑Hybrid and Affinity Approaches (Yuguo Zhang et al.)

  7. In Situ Binding Assay to Detect Myosin‑1c Interactions with Hair‑Cell Proteins (Kelli R. Phillips & Janet L. Cyr)

  8. Ultrastructural Analysis of Kinesin‑Related Motor Proteins During Spermatogenesis (Wan‑Xi Yang)

  9. In Vitro Motility System to Study the Role of Motor Proteins in Receptor‑Ligand Sorting (John W. Murray & Allan W. Wolkoff)

  10. Enrichment and Disassembly of Ectoplasmic Specializations in the Rat Testis (Julian A. Guttman et al.)

  11. Single‑Molecule Observation of Rotation of F₁‑ATPase Through Microbeads (Takayuki Nishizaka et al.)

  12. The Use of FRET in the Analysis of Motor Protein Structure (Andre Kasprzak)

  13. Structure Determination of the Motor Domain of Yeast Kinesin Kar3 by X‑Ray Crystallography (Hee‑Won Park)

  14. High‑Resolution Structural Analysis of the Kinesin‑Microtubule Complex by Electron Cryo‑Microscopy (Keiko Hirose & Linda A. Amos)

  15. Chemical‑Genetic Inhibition of Sensitized Mutant Unconventional Myosins (Ryan L. Karcher et al.)

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Tags: Ann O Sperry, Molecular Motors, Molecular Biology

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