(Ebook) Alcohol Methods and Protocols 1st Edition by Laura E Nagy ISBN 9781588299062 2007938049

(Ebook) Alcohol Methods and Protocols 1st Edition by Laura E Nagy ISBN 9781588299062 2007938049

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ISBN 10: 2007938049
ISBN 13: 9781588299062
Author: Laura E Nagy

(Ebook) Alcohol Methods and Protocols 1st Edition Table of contents:

1 Introduction
2.2 Oral Gavage
3.1 Intraperitoneal Injection
3.2 Oral Gavage
4 Notes
References
A Voluntary Oral-Feeding Rat Model for Pathological Alcoholic Liver Injury
2.2 Assessment of Urine Alcohol
2.6 Immunohistochemical Analysis
2.9 Electrophoretic Mobility Shift Assay
2.11 Western Blotting
2.13 Chymotrypsin (2)
3.1.2.1 Processing of Blood, Liver Samples, and Histological Sections
3.1.2.4 Hematoxylin and Eosin (H&E) Staining
3.2 Biochemical Assay of ALT
3.3.1 Assay on Serum Total 8-Isoprostane Level
3.3.2 Thiobarbituric Acid Reactive Substances (TBARS)
3.3.6 Immunohistochemical Analysis
3.3.7.1.1 Total RNA Extraction
3.3.7.1.3 Preparation of Complementary DNA From RNA
3.3.7.2.1.3 Protein Assay
3.3.7.2.1.4 Measurement of Protein Expression With Western Blotting
3.3.8 Analysis of the DNA-Binding Activity of the Transcription Factors (NF-κB and AP-1) With EMSA
References
Intragastric Ethanol Infusion Model in Rodents
1 Introduction
2.2 Diet
3.1.1 Rat Intragastric Catheter
3.1.2 Mouse Intragastric Catheter
3.2 Infusion Set-up
3.3 Diet Preparation
3.4.2 Mouse Surgery
3.5.1 Rat Intragastric Infusion
3.6 Animal Monitoring
References
A Practical Method of Chronic Ethanol Administration in Mice
1 Introduction
3.1 Overview of Ethanol Administration Methods in Mice
3.2.2 Troubleshooting
3.4.1 Labeling
3.4.2 Ethanol Preparation and Administration
3.5.1 Weight Gain and Practical Starting Weights
3.7.1 Weight Gains
4 Notes
References
Analysis of Ethanol Developmental Toxicity in Zebrafish
1 Introduction
3 Methods
3.3 Embryonic Ethanol Dose Determination
3.4.1 In Vivo Cell Death
3.4.2 Apoptotic Cell Death With TUNEL Analysis
3.5 Global Gene Expression Analysis
3.6 Temporal and Spatial Gene Expression Analysis
3.7 Transient Gene Repression Techniques
References
The Avian Embryo in Fetal Alcohol Research
2.2 Ethanol Treatment
2.5 Ratiometric Imaging of Cai 2+ With Fura-2
3.1 Egg Handling
3.3 Pharmacologic Manipulation of Embryos With Microbead Implants
3.4 Apoptosis Assessment
3.5 Ratiometric Imaging of Cai 2+ With Fura-2
4 Notes
References
Artificial Rearing
1 Introduction
2.1.2 Treatment Diet
2.4 Artificial Rearing Components
3.1.1 Stock Milk Diet
3.2.1.2 Intragastric Cannula
3.2.1.3 Washers
3.2.2 Gastrostomy Surgery
3.3 Artificial Rearing
3.3.2 Determination of Diet Infusion Rate
3.3.4 Care of Artificially Reared Pups
4 Notes
References
Intragastric Intubation of Alcohol During the Perinatal Period
1 Introduction
2.3 Measurement of Blood Alcohol Concentrations (BACs)
3.1 Alcohol Administration During the Prenatal Period
3.3 BACs
4 Notes
References
Human Monocytes, Macrophages, and Dendritic Cells
1 Introduction
2.3 Electrophoretic Mobility Shift Assay for Assaying NF-κB
3.1 Isolation of Peripheral Blood Monocytes
3.2 Generation of Human Monocyte-Derived Macrophages
3.4 Isolation of Plasmacytoid DCs (PDCs)
3.7 Cell Stimulation With Pathogen-Derived Agents
3.9 Isolation of Nuclear Proteins
3.11 Mixed Lymphocyte Reaction
References
Generation and Use of Primary Rat Cultures for Studies of the Effects of Ethanol
2.2 Organotypic Slice Cultures
3.2.1 Surgical Equipment
3.2.3.2 Dissection of the Rat Cortices
3.3.1 Reagents
3.4.1 Equipment
3.4.3.2 Preparation and Culture of Mouse Cortical Slices
3.5.1 Equipment
4.1 Fresh Samples
5 Notes
References

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Tags: Laura E Nagy, Alcohol Methods, Protocols