Yeast functional genomics and proteomics : methods and protocols by Stagljar, Igor instant download
1 online resource (xiii, 300 pages, 4 unnumbered pages of plates) :, Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. The chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls, Includes bibliographical references and index, Comparative genome hybridization on tiling microarrays to detect aneuploidies in yeast / Barry Dion and Grant W. Brown -- Identification of transcription factor targets by phenotypic activation and microarray expression profiling in yeast / Gordon Chua -- SGAM: an array-based approach for high-resolution genetic mapping in Saccharomyces cerevisiae / Michael Costanzo and Charles Boone -- Reporter-based synthetic genetic array analysis: a functional genomics approach for investigating the cell cycle in Saccharomyces cerevisiae / Holly E. Sassi [and others] -- The fidgety yeast: focus on high-resolution live yeast cell microscopy / Heimo Wolinski, Klaus Natter, and Sepp D. Kohlwein -- A genomic approach to yeast chronological aging / Christopher R. Burtner, Christopher J. Murakami, and Matt Kaeberlein -- Chemogenomic approaches to elucidation of gene function and genetic pathways / Sarah E. Pierce [and others] -- Identification of inhibitors of chromatin modifying enzymes using the yeast phenotypic screens / Benjamin Newcomb and Antonio Bedalov -- Exploiting yeast genetics to…
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