Ubiquitin-proteasome protocols by Patterson, Cam; Cyr, Douglas M instant download

Ubiquitin-proteasome protocols by Patterson, Cam; Cyr, Douglas M instant download

1 online resource (xi, 381 pages) :, Annotation, Includes bibliographical references and index, Small-molecule inhibitors of proteasome activity / Maria Gaczynska and Pawel A. Osmulski -- Purification of E1 and E1-like enzymes / Arthur L. Haas -- Assays for RING family ubiquitin ligases / Manabu Furukawa, Paul S. Andrews, and Yue Xiong -- Ubiquitin chain synthesis / Shahri Raasi and Cecile M. Pickart -- Purification of proteasomes, proteasome subcomplexes, and proteasome-associated proteins from budding yeast / David S. Leggett, Michael H. Glickman, and Daniel Finley -- Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome / Chang-Wei Liu [and others] -- Cell-free assay for ubiquitin-independent proteasomal protein degradation / Chaim Kahana and Yuval Reiss, Assays of proteasome-dependent cleavage products / Stefan Tenzer and Hansjörg Schild -- Identification of components of protein complexes / Carol E. Parker [and others] -- Mass spectrometric determination of protein ubiqitination / Carol E. Parker [and others] -- Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol / Robert J. Lee, Ardythe A. McCracken, and Jeffrey L. Brodsky -- Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation / Eric Carlson [and others] -- Deubiquitinating enzyme purifucation, assay inhibitors, and characterization / Nathaniel S. Russell and Keith D. Wilkinson -- Measuring ubiquitin conjugation in cells / Edward G. Mimnaugh and Leonard M. Neckers -- Assays for proteasome assembly and maturation / R. Jürgen Dohmen [and others], N-terminal ubiquitination / Aaron Ciechanover -- Quantitating defective ribosome products / Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell --endoplasmic reticulum-associated protein quality control and degradation: screen for ERAD mutants after ethylmethane sulfonate mutagenesis / Antje Schäfer and Dieter H. Wolf…