Two Component Signaling Systems Volume 423 1st Edition by Melvin I Simon, Brian Crane, Alexandrine Crane ISBN 0080549462 9780080549460

Two Component Signaling Systems Volume 423 1st Edition by Melvin I Simon, Brian Crane, Alexandrine Crane ISBN 0080549462 9780080549460

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Product details:

ISBN 10: 0080549462

ISBN 13: 9780080549460 

Author: Melvin I. Simon, Brian Crane, Alexandrine Crane

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections in Two-Component Signaling Systems, Part B include:

• Structural Approaches
• Reconstitution of Heterogeneous Systems
• Intracellular Methods and Assays
• Genome-Wide Analyses of Two-Component Systems

Table of contents:

Section I: Structural Approaches

• The PICM Chemical Scanning Method for Identifying Domain–Domain and Protein–Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis

• Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics

• Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases

• Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR

• TonB/TolA Amino-Terminal Domain Modeling

• Functional Dynamics of Response Regulators Using NMR Relaxation Techniques

• The Design and Development of Tar-EnvZ Chimeric Receptors

• Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli

• Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes

• A Temperature-Sensing Histidine Kinase—Function, Genetics, and Membrane Topology

• The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules

Section II: Reconstitution of Heterogeneous Systems

• Liposome-Mediated Assembly of Receptor Signaling Complexes

• Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines

• Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers

• Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases

• Genetic Dissection of Signaling Through the Rcs Phosphorelay

Section III: Intracellular Methods and Assays

• In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis

• In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes

• In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays

• A “Bucket of Light” for Viewing Bacterial Colonies in Soft Agar

• Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors

• Single-Cell Analysis of Gene Expression by Fluorescence Microscopy

Section IV: Genome-Wide Analyses of Two-Component Systems

• Two-Component Systems of Mycobacterium tuberculosis—Structure-Based Approaches

• Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon in Staphylococcus aureus

• Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling

• Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays

• Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques

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Tags: Melvin I Simon, Brian R Crane, Alexandrine Crane, Two Component, Signaling, Systems, Volume 423, Methods, Enzymology