Stereo-cell: Spatial enhanced-resolution single-cell sequencing with high-density DNA nanoball-patterned arrays by Sha Liao, Xiaoxi Zhou, Chuanyu Liu, Chang Liu, Shijie Hao, Hongyu Luo ISBN 10.1126/SCIENCE.ADR0475 instant download

Stereo-cell: Spatial enhanced-resolution single-cell sequencing with high-density DNA nanoball-patterned arrays by Sha Liao, Xiaoxi Zhou, Chuanyu Liu, Chang Liu, Shijie Hao, Hongyu Luo ISBN 10.1126/SCIENCE.ADR0475 instant download

Single-cell sequencing technologies have advanced our ated microstructures of large sizes and various shapes; and (v) simulunderstanding of cellular heterogeneity and biological taneous determination of the identity and subcellular distribution of complexity. However, existing methods face limitations in each transcript for RNA sequencing.throughput, capture uniformity, cell size flexibility, and By combining DNA nanoball (DNB)–patterned arrays and in situ technical extensibility.
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We present Stereo-cell, a spatial RNA capture, we previously created spatial enhanced-resolution omics enhanced-resolution single-cell sequencing platform based on sequencing (Stereo-seq), which enables in situ transcriptome profiling high-density DNA nanoball (DNB)–patterned arrays, which of frozen tissue sections placed onto the chip surface (18, 19). The DNB enables scalable and unbiased cell capture at a wide input chip features densely packed nanoballs (~220 nm in diameter, spaced range and supports high-fidelity transcriptome profiling. 500 nm from center to center) that facilitate encapsulation-free Stereo-cell further allows integration with imaging-based capture of both cells and transcripts.
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This design achieves single-cell modalities and multiomics strategies, including spatial resolution in transcript detection, directly mapping cellular immunofluorescence and epitope profiling. This platform is molecular signatures to their native tissue context.also compatible with profiling extracellular vesicles, In this work, we develop and report the spatial enhanced-resolution microstructures, and large cells, whereas its spatial resolution single-cell sequencing (Stereo-cell) based on DNB-patterned arrays. facilitates in situ analysis of cell-cell interactions.