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(Ebook) Proteomics in Practice: A Guide to Successful Experimental Design, Second Edition by Dr. Reiner Westermeier, Dr. Tom Naven, Hans?Rudolf Hopker(auth.) ISBN 9783527319411, 9783527622290, 3527319417, 3527622292

  • SKU: EBN-4311040
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Instant download (eBook) Proteomics in Practice: A Guide to Successful Experimental Design, Second Edition after payment.
Authors:Dr. Reiner Westermeier, Dr. Tom Naven, Hans?Rudolf Hopker(auth.)
Pages:492 pages.
Year:2008
Publisher:Wiley-Blackwell
Language:english
File Size:6.09 MB
Format:pdf
ISBNS:9783527319411, 9783527622290, 3527319417, 3527622292
Categories: Ebooks

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(Ebook) Proteomics in Practice: A Guide to Successful Experimental Design, Second Edition by Dr. Reiner Westermeier, Dr. Tom Naven, Hans?Rudolf Hopker(auth.) ISBN 9783527319411, 9783527622290, 3527319417, 3527622292

Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.Content: Chapter 1 History (pages 1–8): Chapter 2 Critical Points (pages 8–12): Chapter 3 Proteomics Strategies (pages 12–13): Chapter 4 Concept of Experimental Planning (pages 14–15): Chapter 1 Electrophoretic Techniques (pages 19–149): Chapter 2 Liquid Chromatography Techniques (pages 151–213): Chapter 3 Mass Spectrometry (pages 215–272): Chapter 4 Functional Proteomics: Studies of Protein–Protein Interactions (pages 273–277): Chapter none Equipment, Consumables, Reagents (pages 279–410): Chapter 1 Two?Dimensional Electrophoresis (pages 411–428): Chapter 2 Mass Spectrometry (pages 429–431):
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