(Ebook) Protein Kinase C Protocols 1st edition by Alexandra Newton 1588290689 9781588290687

(Ebook) Protein Kinase C Protocols 1st edition by Alexandra Newton 1588290689 9781588290687

Protein Kinase C Protocols 1st edition by Alexandra C. Newton - Ebook PDF Instant Download/DeliveryISBN:  1588290689, 9781588290687 

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Product details:

ISBN-10 :  1588290689 

ISBN-13 : 9781588290687

Author: Alexandra C. Newton 

Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction. Protein Kinase C Protocols covers fundamental methods for studying the structure, function, regulation, subcellular localization, and macromolecular interactions of PKC. Protein Kinase C Protocols is divided into 11 sections representing the major aspects of PKC regulation and function. 

Protein Kinase C Protocols 1st Table of contents:

Part I contains an introduction and a historical perspective on the discovery of PKC by Drs. Yasutomi Nishizuka and Ushio Kikkawa.

Part II describes methods to purify PKC. 

Part III describes the standard methods for measuring PKC activity: its enzymatic activity and its stimulus-dependent translocation from the cytosol to the membrane.

Part IV describes methods for measuring the membrane interaction of PKC in vivo and in vitro. 

Part V provides methodologies and techniques for measuring the ph- phorylation state of PKC, including a protocol for measuring the activity of PKC’s upstream kinase,

Part VI. PDK-1. Novel methods for identifying substrates are described 

Part VII presents protocols for expressing and analyzing the membrane targeting domains of PKC.

Part VIII provides a comprehensive c- pilation of methods used to identify binding partners for PKC.

Part IX describes pharmacological probes used to study PKC.

Part X The book ends with a presentation of genetic approaches to study PKC

Part XI a discussion of approaches used to study PKC in disease.

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Tags: Protein Kinase, Protocols, Alexandra Newton, phospholipid hydrolysis