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15 reviews(Ebook) Principles of Genome Analysis and Genomics 3rd Edition by Sandy B Primrose, Richard Twyman - Ebook PDF Instant Download/Delivery: 9781405101202 ,1405101202
Full download (Ebook) Principles of Genome Analysis and Genomics 3rd Edition after payment
Product details:
ISBN 10: 1405101202
ISBN 13: 9781405101202
Author: Sandy B Primrose, Richard Twyman
(Ebook) Principles of Genome Analysis and Genomics 3rd Edition Table of contents:
CHAPTER 1 Setting the scene: the new science of genomics
Introduction
Physical mapping of genomes
Sequencing whole genomes
Bene •ts of genome sequencing
Outline of the rest of the book
Terminology
Keeping up to date
Suggested reading
Useful websites
CHAPTER 2 The organization and structure of genomes
Introduction
Genome size
Sequence complexity
Introns and exons
Genome structure in viruses and prokaryotes
The organization of organelle genomes
The organization of nuclear DNA in eukaryotes
Suggested reading
Useful websites
CHAPTER 3 Subdividing the genome
Introduction
Fragmentation of DNA with restriction enzymes
Separating large fragments of DNA
Isolation of chromosomes
Chromosome microdissection
Vectors for cloning DNA
Yeast arti •cial chromosomes
P1-derived and bacterial arti •cial chromosomes as alternatives to yeast arti •cial chromosomes
Retro •tting
Choice of vector
Suggested reading
Useful website
CHAPTER 4 Assembling a physical map of the genome
Introduction
Restriction enzyme •ngerprinting
Marker sequences
Hybridization assays
Physical mapping without cloning
Integration of different mapping methods
Suggested reading
Useful websites
CHAPTER 5 Sequencing methods and strategies
Basic DNA sequencing
Modi •cations of chain-terminator sequencing
Automated DNA sequencing
DNA sequencing by capillary array electrophoresis
Basecalling and sequence accuracy
High throughput sequencing
Sequencing strategies
Alternative DNA sequencing methodologies
Suggested reading
Useful websites
CHAPTER 6 Genome annotation and bioinformatics
Introduction
Traditional routes to gene identi •cation
Databases
Overview of sequence analysis
Detecting open-reading frames
Software programs for •nding genes
Using homology to •nd genes
Analysis of non-coding RNA and extragenic DNA
Identifying the function of a new gene
Secondary databases of functional domains
Gene ontology
Analyses not based on homology
Genome annotation
Molecular phylogenetics
Suggested reading
Useful websites
CHAPTER 7 Comparative genomics
Introduction
Orthologues,paralogues and gene displacement
Protein evolution by exon shuf •ing
Comparative genomics of prokaryotes
Comparative genomics of organelles
Comparative genomics of eukaryotes
Other aspects of comparative genomics
Suggested reading
Useful websites
CHAPTER 8 Protein structural genomics
Introduction
Determining gene function by sequence comparison
Determining gene function through conserved protein structure
Approaches to protein structural genomics
Suggested reading
Useful website
CHAPTER 9 Global expression pro •ling
Introduction
Traditional approaches to expression pro •ling
Global analysis of RNA expression
Global analysis of protein expression
Suggested reading
Useful websites
CHAPTER 10 Comprehensive mutant libraries
Introduction
High-throughput systematic gene knockout
Genome-wide random mutagenesis
Suggested reading
Useful websites
CHAPTER 11 Mapping protein interactions
Introduction
Methods for protein interaction analysis
Library-based methods for screening protein interactions
Informatics tools and resources for protein interaction data
Suggested reading
Useful websites
CHAPTER 12 Applications of genome analysis and genomics
Introduction
Theme 1:Understanding genetic diseases of humans
Theme 2:Understanding responses to drugs (pharmacogenomics)
Theme 3:Understanding and combatting bacterial pathogenicity
Theme 4:Getting to grips with quantitative traits
Theme 5:The impact of genomics on agriculture
Theme 6:Developmental genomics
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Tags: Sandy B Primrose, Richard Twyman, Genome Analysis, Genomics
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