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(Ebook) Plant Biotechnology The Genetic Manipulation of Plants 2nd edition by Adrian Slater, Nigel Scott, Mark Fowler 0199282617 9780199282616

  • SKU: EBN-43687150
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Authors:Adrian Slater, Nigel W. Scott, Mark R. Fowler
Pages:400 pages.
Year:2002
Editon:2
Publisher:Oxford University Press
Language:english
File Size:14.93 MB
Format:pdf
ISBNS:9780199282616, 0199282617
Categories: Ebooks

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(Ebook) Plant Biotechnology The Genetic Manipulation of Plants 2nd edition by Adrian Slater, Nigel Scott, Mark Fowler 0199282617 9780199282616

Plant Biotechnology: The Genetic Manipulation of Plants 2nd edition by Adrian Slater, Nigel W. Scott, Mark R. Fowler - Ebook PDF Instant Download/DeliveryISBN:  0199282617, 9780199282616  

Full download Plant Biotechnology: The Genetic Manipulation of Plants 2nd edition after payment.



Product details:

ISBN-10 ‏ : ‎0199282617 

ISBN-13 ‏ : ‎ 9780199282616

Author:  Adrian Slater, Nigel W. Scott, Mark R. Fowler 

Throughout history, humankind has pursued means to improve the yield of crop plants through selective plant breeding and hybridization. Today, genetic manipulation provides a powerful tool for directing plant breeding. But how is genetic manipulation implemented? What benefits can it offer? And what are the broader issues surrounding the use of this technology? The second edition of Plant Biotechnology: the genetic manipulation of plants presents a balanced, objective exploration of the technology behind genetic manipulation, and the application of this technology to the growth and cultivation of plants. The book describes the techniques underpinning genetic manipulation in a clear, lucid manner, and makes extensive use of case studies to illustrate how this influential tool is used in practice. Key themes and strategies are developed using appropriate case studies, which place the science behind plant biotechnology in its broader agricultural context. Online Resource Centre: The Online Resource Centre features the following materials: For lecturers: BL Figures from the book, available to download For students: BL Hyperlinks to the primary literature articles cited in the book to facilitate access to original research papers BL Case studies BL News updates - surveys of significant developments in the field help maintain the currency of the resource BL Web links library

 

Plant Biotechnology: The Genetic Manipulation of Plants 2nd Table of contents:

1 Plant genomes: the organization and expression of plant genes
Introduction
DNA, chromatin, and chromosome structure
Chromatin
An introduction to gene structure and gene expression
Gene structure and expression in a eukaryotic protein-coding gene
Translation
Regulation of gene expression
Chromatin conformation
Gene transcription
RNA modification, splicing, turnover, and transport
Translation
Post-translational modification
Localization
Protein turnover
Conclusions
Implications for plant transformation
Examples of promoter elements used to drive transgene expression
Protein targeting
Heterologous promoters
Genome size and organization
Arabidopsis and the new technologies
Genome-sequencing projects—technology, findings, and applications
Biotechnological implications of the AGI
Crop plant genome sequencing
Summary
Further reading
2 Plant tissue culture
Introduction
Plant tissue culture
Plasticity and totipotency
The culture environment
Plant cell culture media
Plant growth regulators
Culture types
Callus
Cell-suspension cultures
Protoplasts
Root cultures
Shoot tip and meristem culture
Embryo culture
Microspore culture
Plant regeneration
Somatic embryogenesis
CASE STUDY 2.1 Cereal regeneration via somatic embryogenesis from immature or mature embryos
Organogenesis
Integration of plant tissue culture into plant transformation protocols
Summary
Further reading
3 Techniques for plant transformation
Introduction
Agrobacterium-mediated gene transfer
The biology of Agrobacterium
The Ti plasmid
Ti-plasmid features
The process of T-DNA transfer and integration
Step 1. Signal recognition by Agrobacterium
Step 2. Attachment to plant cells
Step 3. Induction of vir genes
Step 4. T-strand production
Step 5. Transfer of T-DNA out of the bacterial cell
Step 6. Transfer of the T-DNA and Vir proteins into the plant cell and nuclear localization
Practical applications of Agrobacterium-mediated plant transformation
CASE STUDY 3.1 Agrobacterium-mediated transformation of tobacco
Transformation
Direct gene-transfer methods
Particle bombardment
CASE STUDY 3.2 Biolistic transformation of rice
Polyethylene glycol-mediated transformation
Electroporation
Silicon carbide fibres: WHISKERSTM
Summary
Further reading
4 Vectors for plant transformation
Introduction
Desirable features of any plasmid vector
Development of plant transformation vectors
Basic features of vectors for plant transformation
Promoters and terminators
Selectable markers
Reporter genes
Origins of replication
Co-integrative and binary vectors
Families of binary vectors
Optimization
Arrangement of genes in the vector
Transgene copy number
Transgene position
Transgene features
Clean-gene technology
Summary
Further reading
5 The genetic manipulation of herbicide tolerance
Introduction
The use of herbicides in modern agriculture
What types of compounds are herbicides?
Strategies for engineering herbicide tolerance
CASE STUDY 5.1 Glyphosate tolerance
CASE STUDY 5.2 Phosphinothricin
Prospects for plant detoxification systems
Commercialization of herbicide-tolerant plants to date
CASE STUDY 5.3 Engineering imidazolinone tolerance by targeted modification of endogenous plant gene
The environmental impact of herbicide-tolerant crops
The development of super-weeds
Summary
Further reading
6 The genetic manipulation of pest resistance
Introduction
The nature and scale of insect pest damage to crops
GM strategies for insect resistance: the Bacillus thuringiensis approach
The use of B. thuringiensis as a biopesticide
Bt-based genetic modification of plants
CASE STUDY 6.1 Resistance of Bt maize to the European corn borer and other pests
The problem of insect resistance to Bt
The environmental impact of Bt crops
The Copy Nature strategy
CASE STUDY 6.2 Cowpea trypsin inhibitor
Insect-resistant crops and food safety
Summary
Further reading
7 Plant disease resistance
Introduction
Plant–pathogen interactions
Prokaryotes
Fungi and water moulds
Viruses
Existing approaches to combating disease
Natural disease-resistance pathways: overlap between pests and diseases
Anatomical defences
Pre-existing protein and chemical protection
Inducible systems
Systemic responses
Biotechnological approaches to disease resistance
Protection against pathogens
Antimicrobial proteins
Transgenic crops for food safety
Induction of HR and SAR in transgenic plants
CASE STUDY 7.1 The BASF potato
Developments for the future
Other transgenic approaches
Future prospects for breeding
CASE STUDY 7.2 Xanthomonas spp.
Summary
Further reading
8 Reducing the effects of viral disease
Introduction
Types of plant virus
RNA viruses
Entry and replication: points of inhibition
How has the agricultural community dealt with viruses?
CASE STUDY 8.1 Developments in the sugar beet industry
The transgenic approach: PDR
Interactions involving viral proteins
CASE STUDY 8.2 Arabis mosaic virus
RNA effects
Some non-PDR approaches
CASE STUDY 8.3 DNA viruses
What has been commercialized in Western agriculture?
Yellow squash and zucchini
Papaya
Potato
Risk
Summary
Further reading
9 Strategies for engineering stress tolerance
Introduction
The nature of abiotic stress
The nature of water-deficit stress
Different abiotic stresses create a water deficit
CASE STUDY 9.1 Glycine betaine production
Targeted approaches to manipulating tolerance to specific water-deficit stresses
Alternative approaches to salt stress
CASE STUDY 9.2 Na+/H+ antiporters improve salt tolerance in transgenic plants
Alternative approaches to cold stress
CASE STUDY 9.3 The COR regulon
Tolerance to heat stress
Secondary effects of abiotic stress: the production of ROS
Strategy 1: Expression of enzymes involved in scavenging ROS
Strategy 2: Production of antioxidants
Summary
Further reading
10 The improvement of crop yield and quality
Introduction
The genetic manipulation of fruit ripening
CASE STUDY 10.1 The genetic manipulation of fruit softening
CASE STUDY 10.2 The genetic modification of ethylene biosynthesis
CASE STUDY 10.3 Modification of colour
CASE STUDY 10.4 Golden Rice
Engineering plant protein composition for improved nutrition
The genetic manipulation of crop yield by enhancement of photosynthesis
Manipulation of light harvesting and the assimilate distribution: phytochromes
Direct manipulation of photosynthesis: enhancement of dark reactions
Summary
Further reading
11 Molecular farming
Introduction
Carbohydrates and lipids
Carbohydrate production
CASE STUDY 11.1 Starch
CASE STUDY 11.2 Polyfructans
Metabolic engineering of lipids
CASE STUDY 11.3 Bioplastics
Molecular farming of proteins
Production systems
CASE STUDY 11.4 The oleosin system: hirudin and insulin production
Medically related proteins
CASE STUDY 11.5 Custom-made antibodies
CASE STUDY 11.6 Edible vaccines
Economic and regulatory considerations for molecular farming
Summary
Further reading
12 Science and society: public acceptance of genetically modified crops
Introduction
Public concerns
The current state of transgenic crops
Who has benefited from these first-generation GM crops?
What will drive the development of the future generations of GM crops?
Concerns about GM crops
Antibiotic-resistance genes
Herbicide resistance and super-weeds
Gene containment
Big business
Food safety
The regulation of GM crops and products
The EU
The USA
Summary
Further reading
13 Beyond genetically modified crops
Introduction
‘Greener’ genetic engineering
Genetic manipulation of complex agronomic traits
Identification of genes associated with desirable traits
Genetic mapping
Quantitative trait loci
Investigating gene function by reverse genetics
Insertional mutagenesis
TILLING
Understanding gene function within the genomic context: functional genomics
Transcriptomics
Proteomics
Interactomics
Metabolomics
Systems biology

 

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Tags: Plant Biotechnology, The Genetic Manipulation, Adrian Slater, Nigel Scott, Mark Fowler

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