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(Ebook) Molecular Cloning A Laboratory Manual 3rd Edition by Joseph Sambrook, David W Russell ISBN 9780879693732 0879693738

  • SKU: EBN-7166210
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Authors:Joseph Sambrook, David W. Russell
Pages:2231 pages.
Year:2001
Editon:3rd
Publisher:CSHL Press
Language:english
File Size:134.22 MB
Format:pdf
ISBNS:9780879693732, 0879693738
Categories: Ebooks

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(Ebook) Molecular Cloning A Laboratory Manual 3rd Edition by Joseph Sambrook, David W Russell ISBN 9780879693732 0879693738

(Ebook) Molecular Cloning A Laboratory Manual 3rd Edition by Joseph Sambrook, David W Russell - Ebook PDF Instant Download/Delivery: 9780879693732 ,0879693738
Full download (Ebook) Molecular Cloning A Laboratory Manual 3rd Edition after payment


Product details:

ISBN 10: 0879693738
ISBN 13: 9780879693732
Author: Joseph Sambrook, David W Russell

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using todays biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

(Ebook) Molecular Cloning A Laboratory Manual 3rd Edition Table of contents:

Volume 1: DNA Preparation and Cloning Basics

  1. Plasmids and Their Usefulness in Molecular Cloning

  2. Bacteriophage and Its Vectors

  3. Working with Bacteriophage M13 Vectors

  4. Working with High-Capacity Vectors

  5. Gel Electrophoresis of DNA and Pulsed-Field Agarose Gel Electrophoresis

  6. Preparation and Analysis of Eukaryotic Genomic DNA

  7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells

Volume 2: DNA Manipulation and Gene Identification

  1. In Vitro Amplification of DNA by the Polymerase Chain Reaction (PCR)

  2. Preparation of Radiolabeled DNA and RNA Probes

  3. Working with Synthetic Oligonucleotide Probes

  4. Preparation of cDNA Libraries and Gene Identification

  5. DNA Sequencing

  6. Mutagenesis

  7. Screening Expression Libraries

Volume 3: Gene Expression and Functional Analysis

  1. Expression of Cloned Genes in Escherichia coli

  2. Introducing Cloned Genes into Cultured Mammalian Cells

  3. Analysis of Gene Expression in Cultured Mammalian Cells

  4. Protein Interaction Technologies

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Tags: Joseph Sambrook, David W Russell, Molecular Cloning, Laboratory Manual

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