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0 reviews(Ebook) Microbiology Laboratory Theory and Application 4th Edition by Michael J Leboffe, Burton E Pierce - Ebook PDF Instant Download/Delivery: 9781617312502 ,1617312509
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ISBN 10: 1617312509
ISBN 13: 9781617312502
Author: Michael J Leboffe, Burton E Pierce
(Ebook) Microbiology Laboratory Theory and Application 4th Edition Table of contents:
SECTION 1: Fundamental Skills for the Microbiology Laboratory
Exercise 1-1: A Comparison of Hand-Cleansing Agents
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 1-1
Basic Growth Media
Exercise 1-2: Nutrient Broth and Nutrient Agar Preparation
Theory
Application
In This Exercise
Materials
Medium Recipes
Preparation of Medium
Data Sheet 1-2
Aseptic Transfers and Inoculation Methods
Exercise 1-3: Common Aseptic Transfers and Inoculation Methods
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 1-3
Exercise 1-4: Streak Plate Methods of Isolation
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 1-4
Exercise 1-5: Spread Plate Method of Isolation
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 1-5
SECTION 2: Microbial Growth
Diversity and Ubiquity of Microorganisms
Exercise 2-1: Ubiquity of Microorganisms
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-1
Exercise 2-2: Colony Morphology
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-2
Exercise 2-3: Growth Patterns on Slants
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-3
Exercise 2-4: Growth Patterns in Broth
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-4
Environmental Factors Affecting Microbial Growth
Exercise 2-5: Evaluation of Media
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 2-5
Aerotolerance
Exercise 2-6: Agar Deep Stabs
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 2-6
Exercise 2-7: Fluid Thioglycollate Broth
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 2-7
Exercise 2-8: Anaerobic Jar
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-8
Effect of Environmental Factors on Bacterial Growth Rate
Exercise 2-9: The Effect of Temperature on Microbial Growth
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-9
Exercise 2-10: The Effect of pH on Microbial Growth
Theory
Application
This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 2-10
Exercise 2-11: The Effect of Osmotic Pressure on Microbial Growth
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 2-11
Control of Pathogens: Physical and Chemical Methods
Exercise 2-12: Steam Sterilization
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-12
Exercise 2-13: The Lethal Effect of UltravioletRadiation on Microbial Growth
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-13
Exercise 2-14: Chemical Germicides:Disinfectants and Antiseptics
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 2-14
SECTION 3: Microscopy and Staining
Exercise 3-1: Introduction tothe Light Microscope
Theory
Application
In This Exercise
Materials
Instructions for Using the Microscope
Köhler Illumination
Procedure
Data Sheet 3-1
Exercise 3-2: Calibration of the Ocular Micrometer
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-2
Exercise 3-3: Microscopic Examination of Eukaryotic Microbes
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-3
Exercise 3-4: Microscopic Examination of Freshwater Microbes
Theory
Application
In This Exercise
Materials
Data Sheet 3-4
Bacterial Structure and Simple Stains
Exercise 3-5: Simple Stains
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-5
Exercise 3-6: Negative Stains
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-6
Differential and Structural Stains
Exercise 3-7: Gram Stain
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-7
Exercise 3-8: Acid-Fast Stains
Theory
Application
In This Exercise
Materials
Procedure: Ziehl-Neelsen (ZN) Method
Procedure: Kinyoun Method
Data Sheet 3-8
Exercise 3-9: Capsule Stain
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-9
Exercise 3-10: Endospore Stain
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 3-10
Exercise 3-11: Wet Mount and Hanging Drop Preparation
Theory
Application
In This Exercise
Materials
Procedure: Wet Mount Preparation
Procedure: Hanging Drop Preparation
Data Sheet 3-11
Exercise 3-12: Flagella Stain
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-12
Exercise 3-13: Morphological Unknown
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 3-13
SECTION 4: Selective Media
A Word about Selective Media
Selective Media for Isolation of Gram-Positive Cocci
Exercise 4-1: Phenylethyl Alcohol Agar
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 4-1
Exercise 4-2: Columbia CNA with 5% Sheep Blood Agar
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 4-2
Exercise 4-3: Bile Esculin Agar
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 4-3
Exercise 4-4: Mannitol Salt Agar
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 4-4
Selective Media for Isolation of Gram-negative Rods
Exercise 4-5: MacConkey Agar
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 4-5
Exercise 4-6: Eosin Methylene Blue Agar (Levine)
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 4-6
Exercise 4-7: Hektoen Enteric Agar
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 4-7
Exercise 4-8: Xylose Lysine Desoxycholate Agar
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 4-8
SECTION 5: Differential Tests
A Word about Biochemical Tests and Acid-Base Reactions
Introduction to Energy Metabolism Tests
Exercise 5-1: Reduction Potential
Theory
Application
In This Exercise
Materials
Media Recipes
Procedure
Data Sheet 5-1
Exercise 5-2: Oxidation–Fermentation Test
Data Sheet 5-2
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Fermentation Tests
Exercise 5-3: Phenol Red Broth
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-3
Exercise 5-4: Methyl Red and Voges–Proskauer Tests
Theory
Application
In This Exercise
Materials
Medium and Reagent Recipes
Procedure
Data Sheet 5-4
Exercise 5-5: ONPG Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-5
Exercise 5-6: Catalase Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-6
Exercise 5-7: Oxidase Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-7
Exercise 5-8: Nitrate Reduction Test
Theory
Application
In This Exercise
Materials
Medium and Reagent Recipes
Procedure
Data Sheet 5-8
Nutrient Utilization Tests
Exercise 5-9: Citrate Utilization Test
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-9
Exercise 5-10: Malonate Utilization Test
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-10
Decarboxylation and Deamination Tests
Exercise 5-11: Decarboxylation Tests
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-11
Exercise 5-12: Phenylalanine Deaminase Test
Theory
Application
In This Exercise
Materials
Medium and Reagent Recipes
Procedure
Data Sheet 5-12
Tests Detecting Hydrolytic Enzymes
Exercise 5-13: Starch Hydrolysis (Amylase Test)
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-13
Exercise 5-14: DNA Hydrolysis (DNase Test)
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-14
Exercise 5-15: Lipid Hydrolysis (Lipase Test)
Theory
Application
In This Exercise
Materials
Media Recipe
Procedure
Data Sheet 5-15
Exercise 5-16: Casein Hydrolysis (Casease Test)
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-16
Exercise 5-17: Gelatin Hydrolysis (Gelatinase Test)
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-17
Exercise 5-18: Urea Hydrolysis (Urease Test)
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 5-18
Exercise 5-19: PYR Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-19
Combination Differential Media
Exercise 5-20: SIM Medium Sulfur Reduction, Indole Production, Motility
Theory
Application
In This Exercise
Materials
Medium and Reagent Recipes
Procedure
Data Sheet 5-20
Exercise 5-21: Triple Sugar Iron Agar / Kligler Iron Agar
Theory
Application
In This Exercise
Materials
Media Recipes
Procedure
Data Sheet 5-21
Exercise 5-22: Lysine Iron Agar
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-22
Exercise 5-23: Litmus Milk Medium
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-23
Antimicrobial Susceptibility Testing
Exercise 5-24: Bacitracin, Novobiocin, and Optochin Susceptibility Tests
Theory
Application
In This Exercise
Materials
Procedure (Per Test)
Data Sheet 5-24
Other Differential Tests
Exercise 5-25: Blood Agar
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-25
Exercise 5-26: CAMP Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-26
Exercise 5-27: Coagulase and Clumping Factor Tests
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-27
Exercise 5-28: Motility Test
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 5-28
Multiple Test Systems
Exercise 5-29: api 20 E Identification System for Enterobacteriaceae and Other Gram-negative Rods
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 5-29
Exercise 5-30: EnteroPluri–Test (Enterotube® II)
Preface
Theory
Application
In This Exercise
Materials
Reagent Recipes
Procedure
Data Sheet 5-30
Bacterial Unknowns Project
Exercise 5-31: Bacterial Unknowns Project
In This Exercise
Materials
Procedure
Data Sheet
SECTION 6: Quantitative Techniques
Exercise 6-1: Standard Plate Count (Viable Count)
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 6-1
Exercise 6-2: Urine Culture
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 6-2
Exercise 6-3: Direct Count (Petroff–Hausser)
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 6-3
Exercise 6-4: Closed-System Growth
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 6-4
Exercise 6-5: Plaque Assay of Virus Titer
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 6-5
Exercise 6-6: Thermal Death Time Versus Decimal Reduction Value
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 6-6
SECTION 7: Medical Microbiology
Exercise 7-1: Snyder Test
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 7-1
Exercise 7-2: Lysozyme Assay
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 7-2
Exercise 7-3: Antimicrobial Susceptibility Test: Disk Diffusion (Kirby-Bauer) Method
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 7-3
Exercise 7-4: Clinical Biofilms
Theory
Application
In This Exercise
Materials
Medium, Stain, and Reagent Recipes
Procedure
Data Sheet 7-4
Exercise 7-5: Morbidity and Mortality Weekly Report (MMWR) Assignment
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 7-5
Exercise 7-6: Epidemic Simulation
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 7-6
Exercise 7-7: Identification of Enterobacteriaceae
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 7-7
Exercise 7-8: Identification of Gram-positive Cocci
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 7-8
Exercise 7-9: Identification of Gram-positive Rods
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 7-9
SECTION 8: Environmental Microbiology
A Word about Trophic Groups
Exercise 8-1: Winogradsky Column
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 8-1
Introduction to the Nitrogen Cycle
Exercise 8-2: Nitrogen Fixation
Theory
Application
In This Exercise
Materials
Medium and Stain Recipes
Procedure
Data Sheet 8-2
Exercise 8-3: Nitrification: The Production of Nitrate
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 8-3
Exercise 8-4: Ammonification
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 8-4
Exercise 8-5: Denitrification: Nitrate Reduction
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 8-5
Sulfur Cycle—Introduction
Exercise 8-6: Photosynthetic Sulfur Bacteria
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 8-6
Exercise 8-7: Chemolithotrophic Sulfur-Oxidizing Bacteria
Theory
Application
In This Exercise
Materials
Medium recipe
Procedure
Data Sheet 8-7
Exercise 8-8: Sulfur-Reducing Bacteria
Theory
Application
In This Exercise
Materials
Medium recipe
Procedure
Data Sheet 8-8
Exercise 8-9: Bioluminescence
Theory
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 8-9
Exercise 8-10: Soil Slide Culture
Theory
Application
In This Exercise
Materials
Stain Recipe
Procedure
Data Sheet 8-10
Exercise 8-11: Soil Microbial Count
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 8-11
Water Quality Testing
Exercise 8-12: Membrane Filter Technique
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 8-12
Exercise 8-13: Multiple Tube Fermentation Methodfor Determining Water Quality
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 8-13
SECTION 9: Food Microbiology
Exercise 9-1: Methylene Blue Reductase Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 9-1
Exercise 9-2: Making Yogurt
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 9-2
Exercise 9-3: Yogurt CFU Density of an Active and Live Culture and Identification of a Yogurt Unknow
Theory
Application
In This Exercise
Materials
Medium Recipes
Procedure
Data Sheet 9-3
SECTION 10: Microbial Genetics
Exercise 10-1: Extraction of DNA from Bacterial Cells
Theory
Application
In This Exercise
Materials
Medium Recipe
Procedure
Data Sheet 10-1
Exercise 10-2: Restriction Digest
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 10-2
Exercise 10-3: Bacterial Transformation: the pGLO™ System
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 10-3
Exercise 10-4: Polymerase Chain Reaction
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 10-4
Exercise 10-5: Ultraviolet Radiation Damage and Repair
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 10-5
Exercise 10-6: Ames Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 10-6
Exercise 10-7: Phage Typing of E. Coli Strains
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 10-7
SECTION 11: Hematology and Serology
Exercise 11-1: Differential Blood Cell Count
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 11-1
Introduction to Antigens and Antibodies
Simple Serological Reactions
Exercise 11-2: Precipitin Ring Test
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 11-2
Exercise 11-3: Double Gel Immunodiffusion
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 11-3
Exercise 11-4: Slide Agglutination
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 11-4
Exercise 11-5: Blood Typing
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 11-5
Exercise 11-6: ELISA for Detecting Antibodies in a Patient’s Sample (The Antibody Capture Method)
Theory
Application
In This Exercise
Materials Needed for this Exercise
Procedure
Data Sheet 11-6
SECTION 12: Eukaryotic Microbes
Eukaryotic Supergroups—A Refresher
Exercise 12-1: Representative Fungi of Medical and Economic Importance
Theory
Division Zygomycota
Rhizopus
Division Ascomycota
Saccharomyces cerevisiae
Candida albicans
Aspergillus
Penicillium
Application
In This Exercise
Materials
Procedure
Data Sheet 12-1
Exercise 12-2: Fungal Slide Culture
Theory
Application
In This Exercise
Materials
Procedure
Data Sheet 12-2
Exercise 12-3: Examination of Simple Eukaryotic Pathogens
Theory
Excavata Pathogens
Giardia lamblia
Trichomonas vaginalis
Trypanosoma brucei
Alveolate Pathogens
Balantidium coli
Plasmodium spp.
Toxoplasma gondii
Unikont Pathogen
Entamoeba histolytica
Application
In This Exercise
Materials
Procedure
Data Sheet 12-3
Exercise 12-4: Parasitic Helminths
Theory
Trematode Parasites Found in Clinical Specimens
Clonorchis sinensis
Paragonimus westermani
Schistosoma mansoni
Cestode Parasites Found in Clinical Specimens
Dipylidium caninum
Echinococcus granulosus
Hymenolepis (Vampirolepis) nana
Taenia spp.
Nematode Parasites Found in Clinical Specimens
Ascaris lumbricoides
Enterobius vermicularis
Hookworms (Ancylostoma duodenale and Necator americanus)
Strongyloides stercoralis
Wuchereria bancrofti
Application
In This Exercise
Materials
Procedure
Data Sheet 12-4
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Tags: Michael J Leboffe, Burton E Pierce, Microbiology Laboratory, Application
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