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ISBN 10: 1107044707
ISBN 13: 9781107044708
Author: Hofmann A, Simon A, Grkovic T, Jones M
Delivering fundamental insights into the most popular methods of molecular analysis, this text is an invaluable resource for students and researchers. It encompasses an extensive range of spectroscopic and spectrometric techniques used for molecular analysis in the life sciences, especially in the elucidation of the structure and function of biological molecules. Covering the range of up-to-date methodologies from everyday mass spectrometry and centrifugation to the more probing X-ray crystallography and surface-sensitive techniques, the book is intended for undergraduates starting out in the laboratory and for more advanced postgraduates pursuing complex research goals. The comprehensive text provides strong emphasis on the background principles of each method, including equations where they are of integral importance to the individual techniques. With sections on all the major procedures for analysing biological molecules, this book will serve as a useful guide across a range of fields, from new drug discovery to forensics and environmental studies.
1 Introduction
1.1 Electromagnetic radiation
1.2 Lasers
Further Reading
2 Spectroscopic methods
2.1 Atomic spectroscopy
2.2 UV/Vis spectroscopy
2.3 Fluorescence spectroscopy
2.4 Luminometry
2.5 Circular dichroism spectroscopy
2.6 Light scattering
2.7 Raman and IR spectroscopy
Further reading
3 Structural methods
3.1 Electron paramagnetic resonance
3.2 Nuclear magnetic resonance
3.3 Electron microscopy
3.4 X-ray crystallography
3.5 X-ray single-molecule diffraction and imaging
3.6 Small-angle scattering
Further reading
4 Physical methods
4.1 Centrifugation
4.2 Mass spectrometry
4.3 Calorimetry
Further reading
5 Surface-sensitive methods
5.1 Surface plasmon resonance
5.2 Quartz crystal microbalance
5.3 Monolayer adsorption
5.4 Atomic force microscopy
Further reading
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a molecular approach
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methods in molecular biology series
Tags: Hofmann A, Simon A, Grkovic T, Jones M, Methods, Molecular
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