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(Ebook) Matrix Metalloproteinase Protocols 1st Edition by J Frederick Woessner Jr, Ian M Clark ISBN 9780896037335 0896037339

  • SKU: EBN-1462896
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Authors:J. Frederick Woessner Jr. (auth.), Ian M. Clark PhD (eds.)
Pages:545 pages.
Year:2001
Editon:1st
Publisher:Humana Press
Language:english
File Size:3.07 MB
Format:pdf
ISBNS:9780896037335, 0896037339
Categories: Ebooks

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(Ebook) Matrix Metalloproteinase Protocols 1st Edition by J Frederick Woessner Jr, Ian M Clark ISBN 9780896037335 0896037339

(Ebook) Matrix Metalloproteinase Protocols 1st Edition by J Frederick Woessner Jr, Ian M Clark - Ebook PDF Instant Download/Delivery: 9780896037335 ,0896037339
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Product details:

ISBN 10: 0896037339
ISBN 13: 9780896037335
Author: J Frederick Woessner Jr, Ian M Clark

From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories.

(Ebook) Matrix Metalloproteinase Protocols 1st Edition Table of contents:

Part I. General Methods

  1. Overview of the Matrix Metalloproteinase Family

  2. Expression and Regulation of MMP Genes

  3. Purification of Matrix Metalloproteinases

  4. Assays for Matrix Metalloproteinase Activity

  5. Substrate Zymography for MMP Detection

  6. Western Blotting and Immunodetection of MMPs

  7. In Situ Zymography

Part II. Inhibitors and Regulation

  1. Tissue Inhibitors of Metalloproteinases (TIMPs): Structure and Function

  2. Methods for Studying TIMP–MMP Interactions

  3. Synthetic Inhibitors of Metalloproteinases

  4. Mechanisms of MMP Regulation

Part III. Specific MMP Protocols

  1. Gelatinases: MMP-2 and MMP-9 Methods

  2. Collagenases: Assays for MMP-1, MMP-8, and MMP-13

  3. Stromelysins and Matrilysins: Detection and Functional Assays

  4. Membrane-Type Matrix Metalloproteinases (MT-MMPs)

  5. Quantification of MMP Gene Expression (RT-PCR and Related Methods)

Part IV. Applications in Cell and Tissue Systems

  1. MMPs in Cell Culture: Assays and Inhibition Studies

  2. Use of Organ Culture for Studying Matrix Degradation

  3. MMPs in Angiogenesis Assays

  4. Animal Models for Investigating MMP Function

  5. In Vivo Imaging of MMP Activity

Part V. Clinical and Pathological Applications

  1. MMPs in Arthritis Research: Detection and Analysis

  2. Matrix Metalloproteinases in Cancer: Protocols for Tumor Studies

  3. Cardiovascular Applications: MMPs in Vascular Biology

  4. Role of MMPs in Wound Healing and Tissue Repair

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Tags: J Frederick Woessner Jr, Ian M Clark, Matrix Metalloproteinase Protocols

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