Mapping effective connectivity by virtually perturbing a surrogate brain by Zixiang Luo & Kaining Peng & Zhichao Liang & Shengyuan Cai & Chenyu Xu & Dan Li & Yu Hu & Changsong Zhou & Quanying Liu ISBN 101038/S4159202502654X instant download

Mapping effective connectivity by virtually perturbing a surrogate brain by Zixiang Luo & Kaining Peng & Zhichao Liang & Shengyuan Cai & Chenyu Xu & Dan Li & Yu Hu & Changsong Zhou & Quanying Liu ISBN 101038/S4159202502654X instant download

Efective connectivity (EC), which refects the causal interactions between brain regions, is fundamental to understanding information processing in the brain; however, traditional methods for obtaining EC, which rely on neural responses to stimulation, are often invasive or limited in spatial coverage, making them unsuitable for whole-brain EC mapping in humans. Here, to address this gap, we introduce Neural Perturbational Inference (NPI), a data-driven framework for mapping whole-brain EC. NPI employs an artifcial neural network trained to model large-scale neural dynamics, serving as a computational surrogate of the brain. By systematically perturbing all regions in the surrogate brain and analyzing the resulting responses in other regions, NPI maps the directionality, strength and excitatory/inhibitory properties of brain-wide EC. Validation of NPI on generative models with known ground-truth EC demonstrates its superiority over existing methods such as Granger causality and dynamic causal modeling. When applied to resting-state functional magnetic resonance imaging data across diverse datasets, NPI reveals consistent, structurally supported EC patterns. Furthermore, comparisons with cortico-cortical evoked potential data show a strong resemblance between NPI-inferred EC and real stimulation propagation patterns. By transitioning from correlational to causal understandings of brain functionality, NPI marks a stride in decoding the brain’s functional architecture and facilitating both neuroscience studies and clinical applications.