(Ebook) Heterologous Expression of Membrane Proteins: Methods and Protocols by Isabelle Mus-Veteau ISBN 9781071623671, 1071623672

(Ebook) Heterologous Expression of Membrane Proteins: Methods and Protocols by Isabelle Mus-Veteau ISBN 9781071623671, 1071623672

This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques usingE. colias a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date,Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Editionserves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.