Guidelines for minimal information on cellular senescence experimentation in vivo by Mikolaj Ogrodnik,1,2,3,* Adams,6 Gorgoulis,16,17,18,19,20 Florian GrubEiji Hara,23,24 Pidder Jansen-Du¨ rr,25 Diana Jurk,26 Sundeep Khosla,27 James L. Kirkland,28,29 Valery Krizhanovsk ISBN 101016/JCELL202405059 instant download

Guidelines for minimal information on cellular senescence experimentation in vivo by Mikolaj Ogrodnik,1,2,3,* Adams,6 Gorgoulis,16,17,18,19,20 Florian GrubEiji Hara,23,24 Pidder Jansen-Du¨ rr,25 Diana Jurk,26 Sundeep Khosla,27 James L. Kirkland,28,29 Valery Krizhanovsk ISBN 101016/JCELL202405059 instant download

SUMMARYCellular senescence is a cell fate triggered in response to stress and is characterized by stable cell-cycle arrest and a hypersecretory state. It has diverse biological roles, ranging from tissue repair to chronic disease.The development of new tools to study senescence in vivo has paved the way for uncovering its physiologicaland pathological roles and testing senescent cells as a therapeutic target. However, the lack of specific andbroadly applicable markers makes it difficult to identify and characterize senescent cells in tissues and livingorganisms. To address this, we provide practical guidelines called ‘‘minimum information for cellular senescence experimentation in vivo’’ (MICSE). It presents an overview of senescence markers in rodent tissues,transgenic models, non-mammalian systems, human tissues, and tumors and their use in the identificationand specification of senescent cells. These guidelines provide a uniform, state-of-the-art, and accessibletoolset to improve our understanding of cellular senescence in vivo.