(Ebook) Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols by Linda Lindberg, Jining Liu, Jan Holgersson (auth.), Alain Beck (eds.) ISBN 9781627033268, 1627033262

(Ebook) Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols by Linda Lindberg, Jining Liu, Jan Holgersson (auth.), Alain Beck (eds.) ISBN 9781627033268, 1627033262

Glyco-engineering is being developed as a method to control the composition of carbohydrates and to enhance the pharmacological properties of monoclonal antibodies (mAbs) and other proteins. In Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols, experts in the field provide readers with production and characterization protocols of glycoproteins and glyco-engineered biopharmaceuticals with a focus on mAbs. The volume is divided in four complementary parts dealing with glyco-engineering of therapeutic proteins, glycoanalytics, glycoprotein complexes characterization, and PK/PD assays for therapeutic antibodies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.