Generation of the Ci1 Reporter Mouse Strain with Enhanced Fluorescence for Tissue Clearing Applications by Manyu Chen & Youqi Li & Juan Huang & Yilong Wang & Hu Zhao instant download

Generation of the Ci1 Reporter Mouse Strain with Enhanced Fluorescence for Tissue Clearing Applications by Manyu Chen & Youqi Li & Juan Huang & Yilong Wang & Hu Zhao instant download

Abstract The advancement of tissue clearing technology Keywords Reporter mouse strain · MScarlet fuorescent has signifcantly propelled neuroscience research. Nevertheprotein · TdTomatoless, the fuorescent proteins used in traditional transgenic mouse strains were not specifcally optimized for tissue clearing procedures, resulting in a substantial decrease in Introductionfuorescent intensity after clearing. In this study, we developed the Ci1 reporter mouse strain (where Ci stands for the Recent advances in tissue clearing technologies have revoChinese Institute for Brain Research, CIBR) based on the lutionized the 3D morphological analysis of biological tisbright red fuorescent protein mScarlet. The Ci1 reporter sues. These methods share common chemical principles of exhibits no fuorescence leakage in various organs or tislipid removal and protein preservation through chemical sue types and can be readily crossed with multiple tissuetreatment [1]. However, the chemical diversity required to specifc Cre lines. Compared to the Ai14 mouse strain, the clear heterogeneous tissues (e.g., bone vs neural tissue) risks Ci1 reporter strain demonstrates lower non-specifc leakage, fuorescent signal degradation, particularly for endogenous stronger fuorescence intensity in diferent tissues, and betfuorescent proteins like tdTomato and GFP [2–4]. This chalter preservation of fuorescence following tissue clearing lenge is amplifed in peripheral nerve studies, where longtreatment. The creation of the Ci1 reporter provides a more range axonal projections traverse multiple tissue types with efective tool for both neuroscience and other biomedical distinct clearing requirements.research applications.Given this situation, there is a need for a new type of