(Ebook) Gene Cloning 1st Edition by Julia Lodge, Peter Lund, Steve Minchin ISBN 9780748765348 0748765344

(Ebook) Gene Cloning 1st Edition by Julia Lodge, Peter Lund, Steve Minchin ISBN 9780748765348 0748765344

(Ebook) Gene Cloning 1st Edition by Julia Lodge, Peter Lund, Steve Minchin - Ebook PDF Instant Download/Delivery: 9780748765348 ,0748765344
Full download (Ebook) Gene Cloning 1st Edition after payment

Product details:

ISBN 10: 0748765344
ISBN 13: 9780748765348
Author: Julia Lodge, Peter Lund, Steve Minchin

(Ebook) Gene Cloning 1st Edition Table of contents:

1 Introduction

1.1 The Beginning of Gene Cloning

1.2 How To Use This Book

1.3 What You Need To Know Before You Read This Book

1.4 A Request From the Authors

Further Reading

2 Genome Organization

2.1 Introduction

2.2 The C-value Paradox

2.3 The Human Genome

2.4 Genomes of Other Eukaryotes

2.5 Bacterial Genomes

2.6 Plasmids

2.7 Viral Genomes

2.8 GC Content

2.9 Physical Characteristics of Eukaryotic Chromosomes

2.10 Karyotype

2.11 Euchromatin and Heterochromatin

2.12 CpG Islands

Questions and Answers

Further Reading

3 Key Tools for Gene Cloning

3.1 Introduction

3.2 Vectors

3.3 Restriction Enzymes

3.4 DNA Ligase

3.5 Transformation

3.6 Purification of Plasmid DNA

3.7 More Restriction Enzymes

3.8 Alkaline Phosphatase

3.9 More About Vectors

3.10 Analyzing Cloned DNA by Restriction Mapping

3.11 Measuring the Size of DNA Fragments

3.12 The Polymerase Chain Reaction and Its Use in Gene Cloning

3.13 How Does PCR Work?

3.14 Designing PCR Primers

3.15 The PCR Reaction

3.16 Uses for PCR Products

3.17 Cloning PCR Products

3.18 Real-time PCR for Quantification of DNA

3.19 Advantages and Limitations of PCR

Questions and Answers

Further Reading

4 Gene Identification and DNA Libraries

4.1 The Problem

4.2 Genomic Library

4.3 Constructing a Genomic Library

4.4 How Many Clones?

4.5 Some DNA Fragments are Under-represented in Genomic Libraries

4.6 Using Partial Digests to Make a Genomic Library

4.7 Storage of Genomic Libraries

4.8 Advantages and Disadvantages of Genomic Libraries

4.9 Cloning Vectors for Gene Libraries

4.10 Vectors Derived from Bacteriophage λ

4.11 Packaging Bacteriophage λ In Vitro

4.12 Cloning with Bacteriophage λ

4.13 Calculating the Titer of your Library

4.14 Cosmid Libraries

4.15 Making a Cosmid Library

4.16 YAC and BAC Vectors

4.17 cDNA Libraries

4.18 Making a cDNA Library

4.19 Cloning the cDNA Product

4.20 Expressed Sequence Tags

4.21 What are the Disadvantages of a cDNA Library?

Questions and Answers

Further Reading

5 Screening DNA Libraries

5.1 The Problem

5.2 Screening Methods Based on Gene Expression

5.3 Complementation

5.4 Immunological Screening of Expression Libraries

5.5. Screening Methods Based on Detecting a DNA Sequence

5.6 Oligonucleotide Probes

5.7 Cloned DNA Fragments as Probes

5.8 Colony and Plaque Hybridization

5.9 Differential Screening

5.10 Using PCR to Screen a Library

Questions and Answers

Further Reading

6 Further Routes to Gene Identification

6.1 How Do We Get From Phenotype to Gene: A Fundamental Problem in Gene Cloning

6.2 Gene Tagging: A Method That Both Mutates and Marks Genes

6.3 A Simple Example of Transposon Tagging in Bacteria: Cloning Adherence Genes from Pseudomonas

6.4 Signature-tagged Mutagenesis: Cloning Bacterial Genes with “Difficult” Phenotypes

6.5 Gene Tagging in Higher Eukaryotes: Resistance Genes in Plants

6.6 Positional Cloning: Using Maps to Track Down Genes

6.7 Identification of a Linked Marker

6.8 Moving From the Marker Towards the Gene of Interest

6.9 Identifying the Gene of Interest

6.10 Cloning of the CF Gene: A Case Study

Questions and Answers

Further Reading

7 Sequencing DNA

7.1 Introduction

7.2 Overview of Sequencing

7.3 Sanger Sequencing

7.4 The Sanger Sequencing Protocol Requires a Single-stranded DNA Template

7.5 Modifications of the Original Sanger Protocol

7.6 Strategies for Sequencing a DNA Fragment

7.7 High-throughput Sequencing Protocols

7.8 The Modern Sequencing Protocol

7.9 Genome Sequencing

7.10 High-throughput Pyrosequencing

7.11 The Importance of DNA Sequencing

Questions and Answers

Further Reading

8 Bioinformatics

8.1 Introduction

8.2 What Does a Gene Look Like?

8.3 Identifying Eukaryotic Genes

8.4 Sequence Comparisons

8.5 Pair-wise Comparisons

8.6 Identity and Similarity

8.7 Is the Alignment Significant?

8.8 What Can Alignments Tell Us About the Biology of the Sequences Being Compared?

8.9 Similarity Searches

8.10 Fasta

8.11 BLAST

8.12 What Can Similarity Searches Tell Us About the Biology of the Sequences Being Compared?

8.13 Multiple Sequence Alignments

8.14 What Can Multiple Sequence Alignments Tell Us About the Structure and Function of Proteins?

8.15 Consensus Patterns and Sequence Motifs

8.16 Investigating the Three-dimensional Structures of Biological Molecules

8.17 Using Sequence Alignments to Create a Phylogenetic Tree

Questions and Answers

Further Reading

9 Production of Proteins from Cloned Genes

9.1 Why Express Proteins?

9.2 Requirements for Protein Production From Cloned Genes

9.3 The Use of E.coli as a Host Organism for Protein Production

9.4 Some Problems in Obtaining High Level Production of Proteins in E. coli

9.5 Beyond E. coli: Protein Expression in Eukaryotic Systems

9.6 A Final Word About Protein Purification

Questions and Answers

Further Reading

10 Gene Cloning in the Functional Analysis of Proteins

10.1 Introduction

10.2 Analyzing the Expression and Role of Unknown Genes

10.3 Determining the Cellular Location of Proteins

10.4 Mapping of Membrane Proteins

10.5 Detecting Interacting Proteins

10.6 Site-Directed Mutagenesis for Detailed Probing of Gene and Protein Function

Questions and Answers

Further Reading

11 The Analysis of the Regulation of Gene Expression

11.1 Introduction

11.2 Determining the Transcription Start of a Gene

11.3 Determining the Level of Gene Expression

11.4 Identifying the Important Regulatory Regions

11.5 Identifying Protein Factors

11.6 Global Studies of Gene Expression

Questions and Answers

Further Reading

12 The Production and Uses of Transgenic Organisms

12.1 What is a Transgenic Organism?

12.2 Why Make Transgenic Organisms?

12.3 How are Transgenic Organisms Made?

12.4 Drawbacks and Problems

12.5 Knockout Mice and Other Organisms: The Growth of Precision in Transgene Targeting

12.6 Is the Technology Available to Produce Transgenic People?

Questions and Answers

Further Reading

13 Forensic and Medical Applications

13.1 Introduction

13.2 Forensics

13.3 DNA Profiling

13.4 Multiplex PCR

13.5 Samples for Forensic Analysis

13.6 Obtaining More Information from DNA Profiles

13.7 Other Applications of DNA Profiling

13.8 Medical Applications

13.9 Techniques for Diagnosis of Inherited Disorders

13.10 Whole Genome Amplification

13.11 Diagnosis of Infectious Disease

13.12 Diagnosis and Management of Cancer

People also search for (Ebook) Gene Cloning 1st Edition:

restriction enzymes in gene cloning
    
research paper on gene cloning
    
role of plasmid in gene cloning
    
recombinant gene cloning
    
report on gene cloning

Tags: Julia Lodge, Peter Lund, Steve Minchin, Gene Cloning