(Ebook) DNA-Encoded Chemical Libraries: Methods and Protocols by David Israel (editor), Yun Ding (editor) ISBN 9781071625446, 1071625446

(Ebook) DNA-Encoded Chemical Libraries: Methods and Protocols by David Israel (editor), Yun Ding (editor) ISBN 9781071625446, 1071625446

This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive,DNA-Encoded Chemical Libraries: Methods and Protocolsis a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.