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(Ebook) Cytokine Protocols by Marc De Ley ISBN 9780896039483, 089603948X

  • SKU: EBN-920410
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Authors:Marc De Ley
Pages:239 pages.
Year:2003
Editon:1
Publisher:Humana Press
Language:english
File Size:2.06 MB
Format:pdf
ISBNS:9780896039483, 089603948X
Categories: Ebooks

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(Ebook) Cytokine Protocols by Marc De Ley ISBN 9780896039483, 089603948X

A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.
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