(Ebook) Culture of Epithelial Cells, Second Edition by R. Ian Freshney(eds.) ISBN 9780471221203, 9780471401216, 0471221201, 0471401218

(Ebook) Culture of Epithelial Cells, Second Edition by R. Ian Freshney(eds.) ISBN 9780471221203, 9780471401216, 0471221201, 0471401218

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.Content:
Chapter 1 Introduction (pages 1–30): R. Ian Freshney
Chapter 2 Cell Interaction and Epithelial Differentiation (pages 31–63): Nicole Maas?Szabowski, Hans?Jurgen Stark and Norbert E. Fusenig
Chapter 3 The Epidermis (pages 65–94): E. Kenneth Parkinson and W. Andrew Yeudall
Chapter 4 Culture of Human Mammary Epithelial Cells (pages 95–135): Martha R. Stampfer, Paul Yaswen and Joyce Taylor?Papadimitriou
Chapter 5 Culture of Human Cervical Epithelial Cells (pages 137–169): Margaret A. Stanley
Chapter 6 Human Prostatic Epithelial Cells (pages 171–194): Donna M. Peehl
Chapter 7 Human Oral Epithelium (pages 195–255): Roland C. Grafstrom
Chapter 8 Normal Human Bronchial Epithelial Cell Culture (pages 257–276): John Wise and John F. Lechner
Chapter 9 Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells (pages 277–301): Leland G. Dobbs and Robert F. Gonzalez
Chapter 10 Isolation and Culture of Intestinal Epithelial Cells (pages 303–335): Catherine Booth and Julie A. O'Shea
Chapter 11 Isolation and Culture of Animal and Human Hepatocytes (pages 337–379): Christiane Guguen?Guillouzo
Chapter 12 Culture of Human Urothelium (pages 381–399): Jennifer Southgate, John R.W. Masters and Ludwik K. Trejdosiewicz
Chapter 13 Other Epithelial Cells (pages 401–436): R. Ian Freshney