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0 reviewsSUMMARYPhosphorus is an essential macronutrient for plants, primarily absorbed from the soil as inorganic phosphate(Pi) through root-located Pi transporters. Despite decades of research into these transporters as targets fordeveloping Pi-efficient crops, their mechanisms for Pi import remain poorly understood. Here, we present thecryo-electron microscopy (cryo-EM) structures of the rice Pi importer OsPHT1;11 in both Pi-bound and unbound forms, characterize its conformational dynamics, and demonstrate how these dynamics contribute toits transport function. Pi is recognized through conserved residues found in plants, with its translocation facilitated by a typical alternating-access mechanism. Single-molecule fluorescence resonance energy transfer(smFRET) analyses show that this transporter undergoes dynamic conformational fluctuations, which aredifferentially linked to its Pi transport capability, with a predominance of extracellular open conformations favoring Pi transport, while more populated intracellular open conformations hinder it. These findings highlightkey conformational determinants of transport activity and provide mechanistic insights into Pi uptake inplants.