(Ebook) Biophysical Chemical and Functional Probes of RNA Structure Interactions and Folding 1st Edition by Daniel Herschlag ISBN 0123743990 9780123743992

(Ebook) Biophysical Chemical and Functional Probes of RNA Structure Interactions and Folding 1st Edition by Daniel Herschlag ISBN 0123743990 9780123743992

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Product details:

ISBN 10: 0123743990

ISBN 13: 9780123743992 

Author:  Daniel Herschlag 

This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells.

• Expert researchers introduce the most advanced technologies and techniques in protein structure and folding
• Includes techniques on tiling assays

Table of contents:

1. Equilibrium hydroxyl radical footprinting

2. Bench-top time-resolved hydroxyl radical footprinting

3. Analysis of hydroxyl radical footprinting gels ‘SAFA’

4. Kinetic modeling of reaction pathways from hydroxyl radical data

5. "BABE" mapping of protein/RNA position

6. Multiplexed….(MOHCA)

7. SHAPE

8. "In-line Probing"

9. NAIM

10. Other NAIM

11. Purification of T7 RNA Polymerase

12. Purification of T4 RNA ligase

13. In vitro transcription of RNA

14. 32P-labeling of RNA
B. BIOPHYSICAL TECHNIQUES

15. Fluorescent labeling of RNAs

16. Assembly of complex RNAs by ‘Moore-Sharp’ ligations

17. Assembly of complex RNAs by ‘Moore-Sharp’ ligations

18. General considerations for smFRET with RNA samples

19. Ion counting

20. ASAXS

21. NLPB

22. smNLPB

23. Gel mobility mapping of junction structure

24. Temperature gradient gels

25. Melting studies

26. Co-transcriptional folding studies

27. Activity assays to follow folding processes

28. 2AP fluorescence

29. EPR measurements of RNA dynamics

30. EPR measurements of RNA dynamics

31. FPA measurements of RNA dynamics

32. EPR methods to study specific metal ion binding sites in RNA

33. Thermodynamic study of site-specific metal ion binding sites in RNA

34. Oligonucleotide hybridization studies of RNA folding pathways

35. Native gel shifts

36. Tiling arrays to assess RNA structure

37. EPR distance measurements in RNA

38. RNA folding in vivo

39. Cleavage of RNAs with ‘restriction DNAzymes’

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Tags: Daniel Herschlag, Biophysical, Chemical, Functional Probes, RNA, Structure, Interactions, Folding