Antibiotic resistance : methods and protocols by Gillespie, S. H instant download
1 online resource (xv, 287 pages) :, At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools, Includes bibliographical references and index, Multiplex polymerase chain reaction detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci / R. Patel, J.R. Uhl and F.R. Cockerill -- Drug susceptibility of mycobacterium tuberculosis through the mycolic acid index / J.M. Viader-Salvadó [and others] -- Micro-well phage replication assay for screening mycobacteria for resistance to rifampin and streptomycin / R. McNerney -- Application of SSCP to identification of resistance mutations / T.D. McHugh -- Quantitative, single-tube, nested, PCR (QSTN-PCR) for determining the antibiotic susceptibility of mycobacterium tuberculosis / N.-T. Jou and M.R. Liebling -- Rapid rifamycin susceptibility testing of small-volume mycrobacterium…
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