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34 reviewsPeripheral nerve transfer is an effective surgical method in restoring motorfunctions of upper limb after peripheral nerve injuries. However, the outcomeof individual function recovery is less predictable. It is crucial to access thelong-term evaluation of function improvement. Here, we developed a fullyimplantable multisite optogenetic stimulation system, which is tailored forwireless, reprogrammable and long-term function evaluation of peripheralnerve plexus with sub nerve resolution. In Thy1-ChR2-EYFP mice, our systeminduced distinct compound muscle action potentials and forelimb movementswhen illuminating different nerve fascicles. Furthermore, we applied the system on a nerve transfer mice model after traumatic brain injury and discoveredinnervation pattern of the transferred and adjacent nerves to multiple musclesconsecutively within 12 weeks after surgery. Our system enabled refined evaluation of electrophysiological and motor functions of peripheral nerveplexus, shining light upon personalized diagnosis and treatment after nerveinjuries or surgeries.