A nucleoporin-associated signaling cascade controls plant immunity via histone modification by Leiwen Pan & Shun Peng & Yuehui Zhang & Huan Chang & Yi Yang & Dongbei Guo & Yuan Guo & Yakun Han & Ting Mao & Yuchen Huang & Shui Wang instant download

A nucleoporin-associated signaling cascade controls plant immunity via histone modification by Leiwen Pan & Shun Peng & Yuehui Zhang & Huan Chang & Yi Yang & Dongbei Guo & Yuan Guo & Yakun Han & Ting Mao & Yuchen Huang & Shui Wang instant download

Genome Biology,Abstractcontributed equally to this work.Background: Plants undergo massive transcriptional reprogramming upon pathogen *Correspondence: infection. The transcription factors SAR DEFICIENT1 (SARD1) and CAM-BINDING [email protected] 60-LIKE G (CBP60g) are master regulators of this process. However, 1 Shanghai Engineering the regulation of SARD1 and CBP60g transcription remains unclear.Research Center of Plant Results: We discover a signaling complex centered on the plant-specifc nucleoporins Germplasm Resources, Shanghai CONSTITUTIVE EXPRESSION OF PR GENES 5 (CPR5) and GUANYLATE-BINDING PROTEINCollaborative Innovation Center of Plant Germplasm Resources, LIKE GTPASE 3 (GBPL3), which critically regulates SARD1 and CBP60g transcription. College of Life Sciences, We establish that the RNA processing complexes NineTeen Complex (NTC) Shanghai Normal University, and CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR (CPSF) act downstream Shanghai 200234, Chinaof CPR5 to activate immunity. A genetic screen identifes GBPL3 and key histone modifcation complex components as suppressors of the autoimmune phenotype in cpr5 mutants, functioning downstream of NTC/CPSF. Transcriptomic and genetic analyses demonstrate that SARD1 and CBP60g are fully responsible for autoimmune activation in cpr5. Crucially, GBPL3 and the histone modifers physically interact, bind directly to the SARD1 and CBP60g loci, and repress their expression. Pathogen infection substantially reduces this binding. Consistently, the active histone mark H3K4me3 at SARD1 and CBP60g is modulated by the CPR5-NTC/CPSF-GBPL3/histone modifers cascade and accumulates signifcantly upon pathogen infection.Conclusions: Our fndings reveal a CPR5-NTC/CPSF-GBPL3/histone modifers signaling cascade that controls the transcription of the SARD1 and CBP60g via histone modifcation, thereby modulating the transcriptional reprogramming during plant immune responses.Keywords: Plant immunity, Nuclear pore complex, C