A battery-free nanofluidic intracellular delivery patch for internal organs by Dedong Yin1,2,3,17, Pan Wang2,17, Yongcun Hao4,5,17, Wei Yue6,17, Xinran Jiang1,17, Kuanming Yao7, ISBN 101038/S4158602508943X instant download

A battery-free nanofluidic intracellular delivery patch for internal organs by Dedong Yin1,2,3,17, Pan Wang2,17, Yongcun Hao4,5,17, Wei Yue6,17, Xinran Jiang1,17, Kuanming Yao7, ISBN 101038/S4158602508943X instant download

The targeted delivery of therapeutics to internal organs to, for example, promote healing or apoptosis holds promise in the treatment of numerous diseases1–4. Currently, the prevailing delivery modality relies on the circulation; however, this modality has substantial efciency, safety and/or controllability limitations5–9. Here we report a battery-free, chipless, soft nanofuidic intracellular delivery (NanoFLUID) patch that provides enhanced and customized delivery of payloads in targeted internal organs. The chipless architecture and the fexible nature of thin functional layers facilitate integration with internal organs. The nanopore–microchannel–microelectrode structure enables safe, efcient and precise electroperforation of the cell membrane, which in turn accelerates intracellular payload transport by approximately 105 times compared with conventional difusion methods while operating under relatively low-amplitude pulses (20 V). Through evaluations of the NanoFLUID patch in multiple in vivo scenarios, including treatment of breast tumours and acute injury in the liver and modelling tumour development, we validated its efciency, safety and controllability for organ-targeted delivery. NanoFLUID-mediated in vivo transfection of a gene library also enabled efcient screening of essential drivers of breast cancer metastasis in the lung and liver. Through this approach, DUS2 was identifed as a lungspecifc metastasis driver. Thus, NanoFLUID represents an innovative bioelectronic platform for the targeted delivery of payloads to internal organs to treat various diseases and to uncover new insights in biology.